Center for Lignocellulose Structure and Formation

About the Center

EFRCs (Energy Frontier Research Centers)

The Center for Lignocellulose Structure and Formation (CLSF) is one of 46 Energy Frontier Research Centers (EFRC) initially established in 2009 by the US Department of Energy to accelerate research to meet critical energy challenges of the 21st century. EFRCs integrate the expertise of multiple leading scientific investigators to enable fundamental research of a scope and complexity that would not be possible with the small group research project. The CLSF received renewed funding in 2014 based both on our achievements to date and the quality of our proposal for future research. More information about the EFRC program can be found at the DOE EFRC website.

Our mission: CLSF is working to dramatically increase our fundamental knowledge of the physical structure of biopolymers in plant cell walls to provide a scientific basis for improved methods of converting biomass into transportation fuels.


The EFRCs are called to be synergistic: our strength is the collaborations between researchers with different backgrounds: biologists, chemists, chemical engineers, computional scientists. Penn State University is the lead institution with partners at North Carolina State University, University of Rhode Island, University of Virginia School of Medicine, Massachsetts Institute of Technology, University of Texas at El Paso, Stony Brook University and Oak Ridge National Laboratory each of which contributes special expertise to the Center. Please visit our People page to learn more about each of our Senior Investigators' research interests.

Lignocellulose Focus

Lignocellulose is the major structural material of plant bodies and constitutes the enormously important biorenewable resource used to make building materials, paper, textiles and many polymer derivatives. At the nanoscale, lignocellulose is a highly versatile composite of three complex biopolymers, namely, crystalline nm-scale fibrils of cellulose which are linked together by less-ordered polysaccharides (such as xylans) and embedded in lignin, a complex and heterogeneous phenolic macromolecule.

Despite its huge economic importance, many questions about how plants can convert soluble sugar into high-strength crystalline cellulose microfibrils and combine them with other polymers to make cell walls with diverse physical properties. Plants lock up the majority of their stored photosynthetically-captured solar energy in their cell walls. Honed by 500 million years of evolution and refinement, the nanoscale construction of the plant cell wall endows it with a remarkable combination of mechanical properties and resistance to enzymatic and chemical attack. Its intricate and complex structure makes the wall recalcitrant to conversion to biofuels, necessitating expensive and energy-intensive pretreatments as an early step for biofuel production. Current pretreatment methods were developed empirically, not based on a nano-scale understanding of cell wall structure. With a deeper understanding of the physico-chemical interactions underlying cell wall structure and its assembly, more efficient methods of wall deconstruction and genetic engineering of cell walls may be developed for improvements in bioenergy production, plant growth and cellulose-based materials.

In addition to its current economic importance as a biomaterial, lignocellulose is also the largest store of renewable solar energy on Earth. DOE established three centers in 2009 to develop cellulosic biomass into an economic transportation fuel. Two of the programs focus on trying to break down cell walls. The third, our Center for Lignocellulose Structure and Function headquartered at Penn State, looks at the problem from the opposite perspective: how cell walls are made in the first place. The idea is that understanding how cell walls are assembled will make it easier for us to take them apart or alter them to suit our needs.

Our Work

CLSF continues to enhance its state-of-the-art transdisciplinary research to uncover the details of plant cell wall structure and cellulose microfibril formation.  The work of our center is organized around these basic questions:

  1. Lignocellulose illustrationHow do plants synthesize para-crystalline cellulose microfibrils?
    1. What are the key structure/function relationships in plant cellulose synthases (CESAs)?
    2. What is the structure and composition of the plant cellulose synthase complex and how does it produce the cellulose microfibril?
  2. How do cellulose and other components form extensible cell walls during growth and stable, reinforced walls at maturity?
    1. How are nano- and meso-scale structure and assembly linked to growth and mechanics in primary cell walls?
    2. What are the nano- and meso-scale details of secondary cell wall structure, and how are they formed during secondary wall synthesis?

The center is comprised of a unique mix of experts in computational modeling, biochemistry, biophysics, biopolymer analytics, cell biology, genetics, molecular biology, polymer physics, synthetic chemistry, and systems biology specifically chosen to work synergisterically to tackle key questions of lignocellulose structure and formation, using both experimental and theoretical (including computational) approaches.


The fundamental knowledge and technical expertise to be developed by the Center is essential for designing novel ways to manipulate plant cell walls, an important step in unlocking the energy-rich cell wall for the next generation of sustainable biofuels and for creating new cellulosic biomaterials with diverse economic applications. Additionally, the understanding of how nature creates this most versatile of biocomposites could be used to create new composites based on different polymers.

More information

About the website

Web design and development services were provided by Patricia Craig. Website updated by Amanda Snyder and Laura Ullrich (Gilliland).